THE NATURE OF COLICIN K FROM PROTEUS MIRABILIS
Open Access
- 1 May 1970
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 131 (5), 1016-1038
- https://doi.org/10.1084/jem.131.5.1016
Abstract
The colicinogenic factor K has been transferred from E. coli K 235 to Proteus mirabilis. The DNA of the colicinogenic Proteus has been shown to contain a small amount of a satellite DNA which presumably harbors the Col K factor. In the presence of mitomycin C the colicinogenic Proteus secretes colicin K into the growth medium. The bacteriocin has been purified by chromatography and obtained as an immunologically homogeneous substance unconjugated with other antigens of the Proteus bacillus. Proteus colicin K is a protein of relatively low molecular weight. It contains all of the usual amino acids except cysteine and is free of lipids and polysaccharides. The bacteriocin can be separated by electrofocusing into two major components. The latter have the same biological properties but differ in their specific electrical charges.Keywords
This publication has 26 references indexed in Scilit:
- Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coliJournal of Molecular Biology, 1968
- EpisomesAnnual Review of Microbiology, 1967
- [Purification of colchicine K].1967
- Evidence for circular DNA forms of a bacterial plasmid.Proceedings of the National Academy of Sciences, 1967
- The DNA of Chloroplasts, Mitochondria, and CentriolesProgress in Nucleic Acid Research and Molecular Biology, 1967
- Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductasesBiochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis, 1966
- THE NUCLEIC ACIDS OF T2, T4, AND T6 BACTERIOPHAGESThe Journal of Experimental Medicine, 1957
- On the presence of sialic acid in certain glycoproteins and in gangliosides.1952
- ELECTROPHORESIS OF PROTEINS ON FILTER PAPERThe Journal of general physiology, 1951
- Antigen — Antibody Reactions In GelsActa Pathologica Microbiologica Scandinavica, 1949