Preparation and partial characterization of sheep erythrocyte-antibody-complement intermediate.

Abstract

A reagent has been prepared which contains C′4, C′2, C′3, C′5, C′6 and C′7 by chromatography of guinea-pig serum on CM-cellulose. With this reagent, when supplemented with additional C′5, EAC′1a,4,2a,3,5,6,7 was prepared from EAC′1a. The reactivity and stability of cells possessing active C′7 sites prepared with this reagent appeared to be the same as EAC′1a,4,2a,3,5,6,7 prepared from EAC′1a,4 and mixtures of individual functionally pure components. The reactivity of cells possessing active C′7 sites has been measured by initial velocity of generation of C′8 sites, and an inhibitor of this reaction, Antrypol, has been described. A method for the estimation of the average number of C′7 sites was investigated and a correlation appears to exist between the average number of sites per cell and the initial velocity of reaction with C′8. Finally, a provisional method for the assay of C′8 and C′9 has been described.