REACTIVITY OF THE EPITHELIAL-CELLS OF THE BOVINE OVIDUCT INVITRO ON THE EXOGENIC, GONADOTROPIC AND STEROID-HORMONES .2. EFFECT OF GONADOTROPIC-HORMONES ON THE AMOUNT OF GLYCOGEN AND OF ACID AND ALKALINE-PHOSPHATASES

Abstract

The effect of certain gonadotropic and steroid hormones on the epithelial cells of the bovine cultured in vitro was investigated. The effect of the hormones on the activity of acid and alkaline phosphatases was observed. Application of hormones to the medium caused an increase of the amount of glycogen in cells. The activity of alkaline phosphatase increased mostly after application of estradiol (0.2 .mu.g), progesterone (2 .mu.g) and the luteinizing hormone (0.1 .mu.g) and the activity of acid phosphatase after the minimum dose of the luteinizing hormone and estradiol and the maximum dose of progesterone. On the basis of morphological and histochemical observations, it was assumed that the epithelial cells of the bovine oviduct cultured in vitro may undergo transformations.