High-Pressure Liquid Chromatographic Determination of Indomethacin in Capsules

Abstract
A high-pressure liquid chromatography method was developed for the quantitative determination of indomethacin, a nonsteroidal antiinflammatory agent, in capsules. The method was based on the utilization of 1-(p-fluorobenzoyl)-5-methoxy-2-methylindol acetic acid as the internal standard of the acetonitrile - 0.1M acetic acid (55:45 vv/v) as the mobile phase. Indomethacin and the internal standard were well resolved and eluted with retention times of 7.5 and 6.5 min. respectively. The applicability of the method is demonstrated.