Evaluation of Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test and Roche PCR-microwell plate hybridization method (AMPLICOR MYCOBACTERIUM) for direct detection of mycobacteria
- 1 January 1996
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 34 (1), 130-133
- https://doi.org/10.1128/jcm.34.1.130-133.1996
Abstract
We evaluated the clinical utility of an rRNA amplification-based Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test (AMTD) system and a PCR-based Roche AMPLICOR MYCOBACTERIUM system for direct detection of Mycobacterium tuberculosis, M. avium, and M. intracellulare. Of the 422 sputum samples from 170 patients, 137 (121 of M. tuberculosis, 14 of M. avium-M. intracellulare complex [MAC], 2 of mycobacterium other than M. tuberculosis or MAC) were culture positive with the Septi-Chek AFB system. One sample of a contaminated culture results was excluded for further analyses. The AMTD system detected all of the 121 samples which grew M. tuberculosis (sensitivity, 100%). Of the 284 culture negative samples, 28 were positive by this system (specificity, 90.1%). After resolution of the discrepant samples, based on a positive history for culture of the patient, the specificity of this system increased to 99.3%. On the other hand, the AMPLICOR system gave a positive result for 132 out of the 135 culture positive samples for M. tuberculosis or MAC (sensitivity, 97.8%). Of the 284 culture-negative samples, 37 were positive by this system (specificity, 87.0%). The specificity for this system after resolution of the discrepant samples increased to 98.9%. The agreement between the results from the AMTD system and the AMPLICOR system was 98.7%. Both of the systems are highly sensitive and specific for detecting M. tuberculosis and/or MAC directly from sputum samples within hours, and they should be recommended for routine use in the clinical microbiology laboratory.Keywords
This publication has 15 references indexed in Scilit:
- Pulmonary Tuberculosis in HIV-Infected Patients in Zaire — A Controlled Trial of Treatment for Either 6 or 12 MonthsNew England Journal of Medicine, 1995
- Comparative study of a biphasic culture system (Roche MB Check system) with a conventional egg medium for recovery of mycobacteriaTubercle and Lung Disease, 1993
- Tuberculosis: Commentary on a Reemergent KillerScience, 1992
- Multidrug-resistant TuberculosisAnnals of Internal Medicine, 1992
- An Outbreak of Tuberculosis with Accelerated Progression among Persons Infected with the Human Immunodeficiency VirusNew England Journal of Medicine, 1992
- Detection ofMycobacterium tuberculosisin Sputum Samples Using a Polymerase Chain ReactionAmerican Review of Respiratory Disease, 1991
- Diagnosis of tuberculosis by DNA amplification in clinical practice evaluationThe Lancet, 1991
- The global tuberculosis situation and the new control strategy of the World Health OrganizationTubercle, 1991
- Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactionsGene, 1990
- Tuberculosis and human immunodeficiency virus infection in developing countriesThe Lancet, 1990