ENHANCEMENT OF X-RAY TRANSFORMATION BY 12-0-TETRADECANOYL-PHORBOL-13-ACETATE IN A CLONED LINE OF C3H MOUSE EMBRYO CELLS

Abstract

A phorbol ester promoting agent, 12-O-tetradecanoylphorbol-13-acetate, enhances X-ray transformation in vitro in a 2-stage fashion similar to that shown previously for UV radiation and chemical carcinogens. In studies with a mouse embryo-derived cell line (C3H/10T1/2 clone 8), there were clear interactive effects between X-radiation and 12-O-tetradecanoylphorbol-13-acetate. These were particularly marked when a minimally transforming X-ray dose (50 or 100 rads) was followed by 12-O-tetradecanoylphorbol-13-acetate treatment beginning immediately after radiation exposure or 48-96 h later.