Fusion of dipalmitoylphosphatidylcholine vesicles at 4.degree.C
- 17 August 1982
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 21 (17), 4126-4132
- https://doi.org/10.1021/bi00260a032
Abstract
Small sonicated dipalmitoylphosphatidylcholine vesicles when incubated at 4.degree. C and high concentrations fuse completely to vesicles about 700-.ANG. diameter in 7 days, and these further fuse to about 950 .ANG. diameter vesicles after 3-4 wk. The 950 .ANG. diameter vesicles are spherical, homogeneous, mostly unilamellar, have an internal aqueous space about 10 times that of small vesicles, and are stable for at least 6 mo. The 950-.ANG. vesicles are characterized by agarose gel chromatography, freeze-fracture EM, trapped volume measurements, differential scanning calorimetry, and diphenylhexatriene fluorescence polarization.This publication has 22 references indexed in Scilit:
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