Inhibition of Formation of Protein-Bound Hydroxyproline by Free Hydroxyproline in Avena Coleoptiles
- 1 September 1967
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 42 (9), 1165-1170
- https://doi.org/10.1104/pp.42.9.1165
Abstract
Free hydroxyproline inhibits the formation of protein-bound hydroxyproline from proline to a considerably greater extent than it does the incorporation of proline into protein of auxin-treated Avena coleoptiles. This inhibition is greater in the wall than in the cytoplasmic fraction. In the absence of auxin, free hydroxyproline exerts little or no inhibition of hydroxyproline formation. Furthermore free hydroxyproline has no effect on respiration, RNA synthesis or the incorporation of leucine into protein. Hydroxyproline is not a general inhibitor of metabolism or protein synthesis in Avena coleoptiles. These results suggest that free hydroxyproline may inhibit auxin-induced cell elongation by blocking the formation or utilization of a particular hydroxyproline-rich protein which must be incorporated into the cell wall during auxin-induced wall extension. - -Author.This publication has 15 references indexed in Scilit:
- Direct incorporation of hydroxyproline into protein of sycamore cells incubated at growth-inhibitory levels of hydroxyproline.Proceedings of the National Academy of Sciences, 1967
- Soluble collagen proline hydroxylase and its substrates in several animal tissues.Proceedings of the National Academy of Sciences, 1966
- Electron microscopical localization of chemical components in the growth zone of Lily pollen tubesProtoplasma, 1966
- SYNTHESIS OF HYDROXYPROLINE IN VITRO BY THE HYDROXYLATION OF PROLINE IN A PRECURSOR OF COLLAGENProceedings of the National Academy of Sciences, 1965
- Hydroxyproline as an Inhibitor of Auxin-induced Cell ElongationNature, 1963
- Effect of Kinetin on Protein & Nucleic Acid Metabolism in Xanthium Leaves During SenescencePlant Physiology, 1962
- A specific method for the analysis of hydroxyproline in tissues and urineAnalytical Biochemistry, 1960
- Ethionine and Auxin-Action in Avena ColeoptilePlant Physiology, 1960
- CHARACTERISTICS OF THE AMINO ACID-INCORPORATING SYSTEM OF LIVER HOMOGENATESJournal of Biological Chemistry, 1952
- SOME ASPECTS OF THE METABOLISM OF HYDROXYPROLINE, STUDIED WITH THE AID OF ISOTOPIC NITROGENJournal of Biological Chemistry, 1949