Cleavage of supercoiled double‐stranded DNA by several ribosome‐inactivating proteins in vitro
- 30 May 1994
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 345 (2-3), 143-146
- https://doi.org/10.1016/0014-5793(94)00421-8
Abstract
Several ribosome-inactivating proteins (RIPs), such as ricin (including its A-chain), luffin, cinnamomin and camphorin, were found to express enzymatic activity to cleave supercoiled double-stranded DNA. In particular, alpha-sarcin, a RIP with a novel ribonuclease activity, was first proved to have this activity. They convert supercoiled DNA into a nicked circular conformation at low concentrations and further into a linear form at high concentrations: they have no effect on linear DNA. Although intact type II RIPs exhibited no RNA N-glucosidase activity, they were detected to cleave supercoiled DNA. Even if ricin A-chain was treated by boiling, its activity on supercoiled DNA was largely retained.Keywords
This publication has 11 references indexed in Scilit:
- Ribotoxin recognition of ribosomal RNA and a proposal for the mechanism of translocationTrends in Biochemical Sciences, 1992
- Trichosanthin, a potent HIV-1 inhibitor, can cleave supercoiled DNAin vitroNucleic Acids Research, 1991
- Ribosomal RNA identity elements for ricin A-chain recognition and catalysisJournal of Molecular Biology, 1991
- Structure of ricin A‐chain at 2.5 ÅProteins-Structure Function and Bioinformatics, 1991
- α-Sarcin cleaves ribosomal RNA at the α-sarcin site in the absence of ribosomal proteinsBiochemical and Biophysical Research Communications, 1988
- The mechanism of action of ricin and related toxic lectins on eukaryotic ribosomes. The site and the characteristics of the modification in 28 S ribosomal RNA caused by the toxins.Journal of Biological Chemistry, 1987
- The site of action of alpha-sarcin on eukaryotic ribosomes. The sequence at the alpha-sarcin cleavage site in 28 S ribosomal ribonucleic acid.Journal of Biological Chemistry, 1982
- The cytotoxic activity of Shigella toxin. Evidence for catalytic inactivation of the 60 S ribosomal subunit.Journal of Biological Chemistry, 1981
- [35] Ricin and ricinus agglutinin, toxic lectins from castor beanMethods in Enzymology, 1978
- Ribosomal Aggregate Engaged in Protein Synthesis: Characterization of the ErgosomeNature, 1963