Isoenzyme of Pyruvate Kinase in Proplastids from Developing Castor Bean Endosperm
- 1 June 1978
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 61 (6), 1037-1039
- https://doi.org/10.1104/pp.61.6.1037
Abstract
Proplastids from developing castor bean (Ricinus communis) endosperm have a pyruvate kinase activity which is extremely unstable on isolation from the organelle. It can be stabilized by 20 mm 2-mercaptoethanol in 20% ethylene glycol. In contrast the soluble pyruvate kinase is stable at 60 C for 10 minutes. The two activities have different pH optima. The soluble and the proplastid activities are eluted from a diethylaminoethyl-Sephadex A-25 sievorptive column at different ionic strengths.Keywords
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