Studies with Detached Lupin Root Nodules in Culture
- 1 November 1975
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 56 (5), 665-670
- https://doi.org/10.1104/pp.56.5.665
Abstract
A method has been developed for culturing detached nitrogen-fixing root nodules of lupin (Lupinus angustifolius L.) on a simple nutrient medium. Under the best conditions devised, the acetylene reduction activity of mature detached nodules was maintained at 10 to 25 nmoles of ethylene hr−1 mg−1 fresh weight for 3 days. Under the same culture conditions, immature nodules increased their acetylene reduction activity from 0.01 nmole or less to about 1 nmole hr−1 mg−1 fresh weight.Keywords
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