High-Resolution Solid-State NMR Applied to Polypeptides and Membrane Proteins
- 13 September 2003
- journal article
- review article
- Published by American Chemical Society (ACS) in Accounts of Chemical Research
- Vol. 36 (11), 858-865
- https://doi.org/10.1021/ar020232y
Abstract
Solid-state NMR provides unique possibilities to study insoluble or noncrystalline molecules at the atomic level. High-resolution conditions can be established by employing magic-angle spinning at ultrahigh magnetic fields. We discuss NMR methods that make use of these experimental improvements and allow for the study of multiply or uniformly [(13)C,(15)N]-labeled polypeptides and proteins. Recent biophysical applications are reviewed.Keywords
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