The Transit of Large Molecules and Particulate Matter Across Individual Endothelial Cells Analyzed in Living Animals With Television Microphotometry

Abstract

The study was undertaken to determine the usefulness of the television system as a quantitative photometric device for securing data from living organs in vivo at the microscopic level of the transit of the azo dyes, Evans and Trypan blue, across selected portions of endothelial cells. The dyes attached to large molecules or as particulate matter coated with large molecules passed through either the cytoplasm or nucleus of endothelial cells. No evidence was found for selective or preponderant passage through the area between endothelial cells.