Induction of transcription from the long terminal repeat of Moloney murine sarcoma provirus by UV-irradiation, x-irradiation, and phorbol ester.
- 1 January 1990
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 87 (1), 36-40
- https://doi.org/10.1073/pnas.87.1.36
Abstract
The long terminal repeat (LTR) of Moloney murine sarcoma virus (Mo-MuSV) was used as a model system to study the stress response of mammalian cells to physical carcinogens. The chloramphenicol acetyltransferase (CAT) gene was inserted between two Mo-MuSV LTRs, and the LTR-CAT-LTR construct was used for virus production and was integrated into the genome of NIH 3T3 cells in the proviral form. This construct was used to assure that the integrated CAT gene was driven by the promoter of the LTR. Expression of the CAT gene was stimulated 4-fold by UV irradiation, and the peak of activity was observed at 18 hr. In contrast, stimulation of the CAT expression after x-irradiation was 2-fold and occurred at 6 hr. Phorbol myristate acetate also stimulated CAT activity 4-fold with a peak at 6 hr. Down-regulation of protein kinase C blocked totally the response to x-irradiation but only partially the response to UV. The protein kinase inhibitor H7 blocked the response to treatment by UV, x-ray, and phorbol ester.This publication has 34 references indexed in Scilit:
- Induction of metallothionein and other mRNA species by carcinogens and tumor promoters in primary human skin fibroblasts.Molecular and Cellular Biology, 1986
- Expression of a mouse long terminal repeat is cell cycle-linked.Proceedings of the National Academy of Sciences, 1985
- Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the geneNucleic Acids Research, 1985
- Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide-dependent protein kinase and protein kinase CBiochemistry, 1984
- Induction of endogenous avian tumor virus gene expression by pyrrolizidine alkaloidsChemico-Biological Interactions, 1984
- Induction of retrovirus gene expression by aflatoxin B1 and 2-acetylaminofluoreneMutation Research Letters, 1983
- Expression of human alpha-tubulin genes: interspecies conservation of 3' untranslated regions.Molecular and Cellular Biology, 1983
- Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirusCell, 1983
- Construction and characterization of the chloramphenicol-resistance gene cartridge: A new approach to the transcriptional mapping of extrachromosomal elementsGene, 1982
- The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.Proceedings of the National Academy of Sciences, 1982