Polyacrylamide Gel Analysis of High Molecular Weight Ribonucleic Acid from Etiolated and Green Cucumber Cotyledons
- 1 July 1970
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 46 (1), 81-85
- https://doi.org/10.1104/pp.46.1.81
Abstract
Cucumis sativus L. seeds and 5-day-old dark-grown cotyledons contain 25 and 18 S cytoplasmic ribosomal RNAs as main components. The major increase in nucleic acid content in both green and etiolated cotyledons occurs between days 5 and 7 of germination. This increase is characterized by an important synthesis of 23 and 16 S plastid (chloroplast and proplastid) ribosomal RNAs. Proplastid RNA synthesis appears to continue for a longer period in the dark-grown cotyledons, despite a total RNA content considerably less than in the light-grown cotyledons.The nonribosomal distribution of the chloroplast and proplastid ribosomal RNAs observed in all cases (after extraction and fractionation) results from the lability of the 23 S component. This degradation increases if the chloroplasts and proplastids are isolated prior to extraction of their nucleic acid.Keywords
This publication has 20 references indexed in Scilit:
- Studies on Spinach Chloroplast and Nuclear DNA Using Large-scale Tissue PreparationsPlant Physiology, 1969
- Ribonuclease Activity Associated With Ribosomes of Zea maysPlant Physiology, 1968
- Synthesis and Stability of Chloroplast Ribosomal—RNA'sPlant Physiology, 1968
- Function of chloroplast DNA. I. Hybridization studies involving nuclear and chloroplast DNA with RNA from cytoplasmic (80S) and chloroplast (70S) ribosomes.Proceedings of the National Academy of Sciences, 1968
- Electrophoretic separation of viral nucleic acids on polyacrylamide gelsJournal of Molecular Biology, 1967
- CHARACTERIZATION OF CYTOPLASMIC AND CHLOROPLAST POLYSOMES IN PLANTS: EVIDENCE FOR THREE CLASSES OF RIBOSOMAL RNA IN NATUREProceedings of the National Academy of Sciences, 1967
- The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresisBiochemical Journal, 1967
- The isolation of ribonucleic acid from plant, bacterial or animal cellsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966
- The Incorporation of Amino Acids into Protein by Cell-free Extracts from Tobacco Leaves*Biochemistry, 1964
- CYTOCHEMICAL STUDIES CONCERNING THE OCCURRENCE AND DISTRIBUTION OF RNA IN PLASTIDS OF ZEA MAYS The Journal of cell biology, 1963