Deposition of the terminal C5b‐9 complement complex on erythrocytes by human red cell autoantibodies

Abstract

A radioassay for the detection of complement activating autoantibodies (autohaemolysins) in patients with autoimmune haemolytic anaemia (AIHA) is described. The method is based on immunoradiometric quantitation of the cytolytic C5b‐9 complement complex following its antibody‐dependent deposition on red blood cells (RBC). The use of affinity‐purified, radiolabelled antibodies directed against the neoantigens of the C5b‐9 complex ensured specificity of the test which proved more sensitive than conventional haemolytic assays and was not subject to disturbances by haemolytic sera. The results obtained in 70 patients with various forms of AIHA (warm type (N= 45); cold type (N= 22); Donath‐Landsteiner type (N= 3)) support the prevailing assumption that autohaemolysins can trigger the complement cascade to completion. Since intact RBC from patients with detectable autohaemolysins carried C3/C4 components but never C5b‐9, it is inferred that regulatory mechanisms operate in vivo at the level of C4b/C3b inactivation to arrest the cascade and rescue the autologous cells.