Isolation and characterization of Saccharomyces cerevisiae mutants defective in glycerol catabolism
- 1 March 1977
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 129 (3), 1335-1342
- https://doi.org/10.1128/jb.129.3.1335-1342.1977
Abstract
Mutants of the yeast S. cerevisiae that are defective in the catabolism of glycerol were isolated, and 2 types of mutants were obtained. One type was deficient in glycerol kinase [EC 2.7.1.30] activity, but the other type was deficient in sn-glycerol 3-phosphate dehydrogenase [EC 1.1.99.5] activity. Genetic analysis indicated that each mutant strain owed its phenotype to a single nuclear mutation and that the 2 mutations were complementary. The mutations were not linked to each other or to any of 10 loci tested. Neither mutation was centromere linked. Possible mechanisms for the regulation of these enzymes were tested by growing the parental strain in the presence of various C sources.This publication has 30 references indexed in Scilit:
- GLYCEROL DISSIMILATION AND ITS REGULATION IN BACTERIAAnnual Review of Microbiology, 1976
- Intracellular localization and properties of glycerokinase and glycerophosphate dehydrogenase in Neurospora crassaArchives of Biochemistry and Biophysics, 1975
- Etude de deux mutants du métabolisme du glycérol chez Bacillus subtilisCanadian Journal of Microbiology, 1972
- Purification of the membrane-bound and pyridine nucleotide-independent L-glycerol 3-phosphate dehydrogenase from Escherichia, coliBiochemical and Biophysical Research Communications, 1972
- Control of permeation to glycerol in cells of EscherichiacoliBiochemical and Biophysical Research Communications, 1968
- Glycerol Metabolism in YeastsEuropean Journal of Biochemistry, 1968
- Genetic control of the l-α-glycerophosphate system in Escherichia coliJournal of Molecular Biology, 1968
- A radiochemical enzymatic activity assay for glycerol kinase and hexokinaseBiochimica et Biophysica Acta (BBA) - Enzymology, 1967
- Feedback Inhibition of Glycerol Kinase, a Catabolic Enzyme in Escherichia coliScience, 1966
- An Enrichment Method for Auxotrophic Yeast Mutants using the Antibiotic ‘Nystatin’Nature, 1966