Inhibition of de novo synthesis of FSH by the Sertoli Cell Factor (SCF)
- 1 August 1978
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 103 (2), 644-647
- https://doi.org/10.1210/endo-103-2-644
Abstract
The effects of SCF, a substance produced by isolated rat Sertoli cells in culture, on the synthesis of FSH [follicle stimulating hormone] and LH [luteinizing hormone] were studied in vitro. Anterior pituitaries from adult male rats were organ-cultured 24-48h in the presence and absence of SCF in the culture medium. The incorporation of 3H-leucine into FSH and LH was determined following 6h incubation with this labelled precursor and immunoprecipitation with specific FSH or LH antibodies. Total FSH and LH were measured by radioimmunoassay. SCF can selectively reduce the incorporation of 3H-leucine into immunoprecipitable FSH without decreasing the incorporation into LH.This publication has 5 references indexed in Scilit:
- The Effects of Testosterone Propionate and Estradiol Benzoate on the in vitro Synthesis of FSHBiology of Reproduction, 1977
- Secretion of an FSH-Inhibiting Factor by Cultured Sertoli CellsEndocrinology, 1976
- DIFFERENCES OF THE EFFECTS OF TESTOSTERONE PROPIONATE ON THE PRODUCTION OF LH AND FSHActa Endocrinologica, 1976
- Pituitary Gonadotropin Content of Aspermatogenic Guinea PigsExperimental Biology and Medicine, 1968
- Pituitary “Total” Gonadotropins, FSH and LH in Orchiectomized or Cryptorchid RatsEndocrinology, 1966