Modification of Cardiac and Smooth Muscle Myosins with 2,4,6-Trinitrobenzenesulfonate

Abstract
Myosins purified from cardiac (porcine heart) and smooth (chicken gizzard) muscles were modified with 2,4,6-trinitrobenzenesulfonate (TNBS) and the effects on the kinetic properties of myosin ATPase [EC 3.6.1.3] were studied. The following results were obtained. The above findings are compared with those previously obtained for skeletal muscle myosin [Miyanishi, T., Inoue, A., & Tonomura, Y. (1979) J. Biochem, 85, 747–753], and the structural and functional differences among the myosins derived from skeletal, cardiac, and smooth muscles are discussed.