Detection of Hb-Papio B, a Silent Mutation of the Baboon β Chain, by High Performance Liquid Chromatography. Improved Procedures for the Separation of Globin Chains by Hplc
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in Hemoglobin
- Vol. 6 (5), 451-464
- https://doi.org/10.3109/03630268209083759
Abstract
A silent mutant of the .beta. chain of the baboon (P. cynocephalus) was detected by high performance liquid chromatography [HPLC] of the globin chains. The substitution is .beta.13 Ala .fwdarw. Thr. The inclusion of nonylamine in the developers for HPLC results in an improved separation of baboon globin chains and in the complete resolution of the human G.gamma., A.gamma. and A.gamma.T chains and some common human variant .beta. chains.Keywords
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