Time-resolved Fluoroimmunoassay in the Detection of Plant Viruses
- 1 May 1987
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 68 (5), 1423-1428
- https://doi.org/10.1099/0022-1317-68-5-1423
Abstract
Time-resolved fluoroimmunoassay (TR-FIA) was used to detect potato virus Y in potato leaf extracts diluted at least 106-fold, and in extracts of secondarily infected dormant tubers diluted 10- or 102-fold depending on the virus strain used. TR-FIA was also used to detect potato virus X in extracts of secondarily infected tubers diluted up to 106-fold. The response in the TR-FIA using a two-incubation method was linearly related to the virus concentration in a series of dilutions and the method can therefore be used for quantitative analysis. TR-FIA was found to be five to 100 times more sensitive than conventional double antibody sandwich ELISA.Keywords
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