SEPARATION OF RACEMATES BY CAPILLARY ZONE ELECTROPHORESIS BASED ON COMPLEXATION WITH CYCLODEXTRIN DERIVATIVES

Abstract

Unmodified and methylated cyclodextrins (CDs) were used as chiral additives to separate dansylamino acid enantiomers by capillary zone electrophoresis (CZE). Unmodified β- or γ-CD showed the good enantioselectivity. Di- or trimethylation of β-CD reduced the chiral recognition. On the other hand, trimethylation of α-CD brought about the appearance of the enantioselectivity. The reverse in the migration order of the enantiomers was observed after the modifications.