Trehalase activation in yeasts is mediated by an internal acidification
- 1 January 1986
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 154 (2), 247-251
- https://doi.org/10.1111/j.1432-1033.1986.tb09389.x
Abstract
It has been reported that the addition of glucose, uncouplers and nystatin to yeast cells grown in a sugar-free medium causes trehalase activation; it has been postulated that this activation might be mediated by the deplorization of the plasma membrane. It this article the values of membrane potential and pH gradient across the plasma membrane of S. cerevisiae have been determined under the same conditions as those in which trehalase is activated. Membrane potential was evaluated from the distribution of triphenylmethylphosphonium, the pH gradient from the distribution of benzoic acid across the plasma membrane. When the effect of several agents on the two components of the electrochemical proton gradient across the plasma membrane of ethanol-grown yeast cells were studied, under trehalase activation conditions, the following observations were made. (a) The addition of glucose activated trehalase and caused internal acidification of the cells, but had practically no effect on the membrane potential. (b) The addition of of 200 mM KCl depolarized the cell membrane but did not affect the internal pH, nor trehalase activity. (c) Although carbonyl cyanide m-chlorophenylhydrazone depolarized the cells at external pH 6.0 and 7.0, it only activated trehalase at an external pH 6.0, leading to the acidification of the internal medium at external pH 6.0 and 7.0, but only activated trehalase at external pH 6.0, causing acidification of the cell interior at this pH. (e) Activation of trehalase was also observed when the internal acidification was caused by addition of a weak acid such as acetate. It is concluded that trehalase activation is mediated by an intracellular acidification and is independent of the membrane potential.This publication has 31 references indexed in Scilit:
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