Transformation of normal human skin fibroblasts by 1-nitropyrene and 6-nitrobenzo [a] pyrene

Abstract

The potent mutagens, 1-nitropyrene and 6-nitrobenzo[a]-pyrene, are shown to induce the transformation of cultured normal human diploid fibroblasts to a state of anchorage- independent growth and cellular invasiveness. The transformation frequencies are greatly enhanced by addition of bovine milk xanthine oxidase, a mammalian nitroreductase.