Studies on the Development of Metacyclic Trypanosoma brucei sspp. Cultivated at 27°C with Insect Cell Lines1
- 1 November 1987
- journal article
- research article
- Published by Wiley in The Journal of Protozoology
- Vol. 34 (4), 372-377
- https://doi.org/10.1111/j.1550-7408.1987.tb03195.x
Abstract
When transformed procyclic trypanosomes of three stocks of Trypanosoma brucei brucei and one stock of T. b. rhodesiense were grown at 27.degree. C in 25-cm2 flasks containing Anopheles gambiae cells, some of them developed into forms infective for mice. Infectivity titrations on trypanosome suspensions revealed that up to 2.8 .times. 105 metacyclic forms per ml could be produced, and the cultures remained infective for varying periods of up to 72 days when they were terminated. Of the various culture media tested, a mixture of three volumes of trypanosome medium and one volume of Anopheles medium was the most successful. Control cultures of trypanosomes grown in medium without cells were generally not infective, but two of the stocks gave rise to a few sporadic infections. Trypanosome populations could be subpassaged in the Anopheles cell cultures without loss of infectivity. Metacyclic forms separated from infective cultures by DEAE-cellulose columns had a surface coat.Keywords
This publication has 20 references indexed in Scilit:
- Infectivity ofTrypanosoma rhodesienseCultivated at 28°C with Various Tsetse Fly Tissues1The Journal of Protozoology, 1986
- Antigenic Analysis By Immunofluorescence of In Vitro‐Produced Metacyclics of Trypanosoma Brucei and Their Infections In Mice*The Journal of Protozoology, 1980
- The Separation and Structure of Infective Trypanosomes From Cultures of Trypanosoma Brucei Grown In Association With Tsetse Fly Salivary Glands*The Journal of Protozoology, 1980
- Infectivity of Trypanosoma brucei Cultivated at 28 C with Tsetse Fly Salivary Glands*The Journal of Protozoology, 1979
- Infectivity Reacquisition by Trypanosoma brucei brucei Cultivated with Tsetse Salivary GlandsScience, 1977
- New Culture Medium for Maintenance of Tsetse Tissues and Growth of Trypanosomatids*The Journal of Protozoology, 1977
- Differentiation of larval drosophila eye‐antennal Discs in VitroJournal of Experimental Zoology, 1964