Cultivation of Yeast in Earle's "L" Strain Mouse Cells in vitro.

Abstract

Earle''s "L" strain mouse cells grown on glass, were inoculated with the yeast cell phase of Histoplasma capsulatum. In stained prepns. it is shown that numerous yeast cells develop intracellularly. The infected animal cells can be subcultured. This system should prove satisfactory in the cultivation of other pathogens, including fungi and also provide a method of studying the effects of therapeutic agents.