Cytochemical Studies on the Intranuclear Inclusion of Herpes Simplex

Abstract

Histochemical studies on the inclusion body of herpes simplex in the chorioallantois at various intervals after infection indicate that the formation of the inclusion is a dynamic process involving many stages which present different morphologic and tinctorial characteristics. In the early inclusion a distinct accumulation of nucleoprotein of the desoxyribose type is present. In the older inclusions presenting the “classical Type A” appearance nucleoprotein is no longer present within the nucleus. An increase in protein synthesis within infected cells is suggested by the very large size of the nucleolus immediately prior to the formation of the inclusion body and by the presence of alkaline phosphatase in the inclusion body.