The Isolation of Migration Inhibition Factor

Abstract
Migration inhibition factor (MIF) has been separated from the supernates of tuberculin stimulated lymphocytes and from fetal calf serum by an affinity column of fucosamine bound to agarose beads. A glycoprotein with the characteristics of biologically active MIF and a molecular weight of approximately 50,000 Daltons was obtained by this one stage purification procedure.