Purification of β-acetylglucosaminase and β-galactosidase from ram testis
- 1 February 1966
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 98 (2), 405-409
- https://doi.org/10.1042/bj0980405
Abstract
The presence of [beta]-galactosidase (EC 3.2.1.23) in an acetic acid extract of ram testis is reported. Some properties of the crude enzyme preparation were studied. The purification of [beta]-acetylglucosaminase (EC 3.2.-1.30) and of [beta]-galactosidase from the ramtestis extract by (NH4)2SO4 precipitation and chromatography on a carboxymethyl-cellulose column is described. The final purifications of the separated enzymes achieved were for the [beta]-acetylglucosaminase 35 times and for the [beta]-galactosidase 99 times. The possibility of using diethylaminoethyl-cellulose and Sephadex G-200 to purify the enzymes was investigated.This publication has 10 references indexed in Scilit:
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