Application of the Paired Radioiodine-Labeled Antibody Technique (Prilat) to the Detection of Adenovirus 12 Tumor (T) Antigen

Abstract

The paired radioiodine-labeled antibody technique (PRILAT) was used to detect adenovirus-12 (Ad-12) T antigen in Ad-12-infected HEp 2 cells and in Ad-12 hamster tumor cells. With appropriate absorption of the paired radiolabeled globulins the PRILAT proved to be highly specific. In comparative studies the PRILAT was 100 to 200 times more sensitive than complement-fixation and 10 times more sensitive than IF. The PRILAT permitted direct calculation of the micrograms of labeled globulins, immune and nonimmune, fixed by each control and test specimen. The method was employed to follow the synthesis of Ad-12 T antigen in synchronously infected HEp 2 cells. De novo synthesis was detected 8 hr post-infection (p.i.) in uninhibited and in cytosine arabinoside-inhibited cultures. Significant specific evanescent reactions that occurred 4 hr p.i. were believed to be due to P antigen released from infecting virions. Maximum T-antigen concentration was obtained 36 hours p.i. Shut-off appeared to occur sooner in the presence of viral DNA synthesis.