Glyceraldehyde, but not cyclic AMP‐stimulated insulin release is preceded by a rise in cytosolic free Ca2+
- 5 November 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 177 (1), 17-22
- https://doi.org/10.1016/0014-5793(84)80972-2
Abstract
The dynamics of changes in membrane potential, cytosolic free Ca2+ [Ca2+]i and immunoreactive insulin release were assessed in RINm5F cells. Membrane depolarization and a rise in [Ca2+]i preceded the stimulation of insulin release by D-glyceraldehyde. Forskolin, which raised the cellular cyclic AMP levels, stimulated insulin release without changing membrane potential or [Ca2+]i. It is concluded that cyclic AMP acts on insulin release not by mobilizing Ca2+ but by another, as yet undefined, mechanism.Keywords
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