Determination of the rates of synthesis and degradation of adenosine 3′,5′-cyclic monophosphate in Escherichia coli CRP− and CRP+ strains
- 1 September 1978
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 56 (9), 849-852
- https://doi.org/10.1139/o78-130
Abstract
A method for estimating the rates of synthesis and degradation of cyclic cAMP in E. coli during balanced growth was developed. This method showed that an E. coli CRP- mutant 5333 (deficient for cAMP receptor protein) synthesizes cAMP about 25 times faster than does its CRP+ parent 1100. This accounts for the abnormally high intracellular and extracellular cAMP accumulation in 5333.This publication has 3 references indexed in Scilit:
- Effects of the spoT and relA Mutation on the Synthesis and Accumulation of ppGpp and RNA during Glucose StarvationCanadian Journal of Biochemistry, 1978
- Evidence against the involvement of adenosine 3′,5′-cyclic monophosphate in glucose inhibition of β-galactosidase induction in Bacillus megateriumCanadian Journal of Biochemistry, 1976
- Effects of crp mutations on adenosine 3',5'-monophosphate metabolism in Salmonella typhimuriumJournal of Bacteriology, 1976