In vitro Binding of Pasteurella multocida Cell Wall Preparations to Tracheal Mucus of Cattle and Swine and to a Tracheal Epithel Cell Wall Preparation of Cattle

Abstract

Outer membrane preparations of various Pasteurella isolates (Pasteurella multocida and some other Pasteurella species) from cattle and swine were extracted by N-lauryl-sarcosine sodium salt. Capsular extracts were prepared by heat treatment. Both preparations bound to epithel cell wall preparations (ECW) of trachea from cattle and to tracheal mucus of cattle and swine. Binding was demonstrated by an enzyme-linked immunosorbent assay (ELISA). Distinct high adherence values were shown by the greater part of membrane preparations of mucoid Pasteurella strains, especially when originating from cattle.