Ein Verfahren zur quantitativen Isolierung reiner Sphingomyeline aus Menschen‐ und Rattenhirn
- 1 January 1963
- journal article
- research article
- Published by Wiley in Helvetica Chimica Acta
- Vol. 46 (2), 601-604
- https://doi.org/10.1002/hlca.19630460220
Abstract
A chromatographic method for the isolation of sphingomyelines from human and rat brain is reported. After isolation of the total lipids we first separate lecithine and sphingomyeline, on a silicagel column. Afterwards lecithine is split by transesterification with sodium methoxide. The cleavage products are separated from sphingomyeline on a silicagel column. Sphingomyeline is then purified on an alumina column. It shows an N: P relation of 2:1, is free of carbohydrates and ester linkages and can be obtained in a yield of 87%.This publication has 13 references indexed in Scilit:
- Neuere Ergebnisse auf dem Gebiet der Isolierung und Analytik von Phosphatiden und GlykolipidenFette, Seifen, Anstrichmittel, 1960
- Synthetic Studies on Sphingolipids. IV. The Synthesis of SphingomyelinJournal of the American Chemical Society, 1959
- Separation and composition of the phospholipids of ox heartBiochemical Journal, 1958
- A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUESJournal of Biological Chemistry, 1957
- THE DETERMINATION OF PHOSPHORUS AND PHOSPHATASE WITH N-PHENYL-p-PHENYLENEDIAMINEJournal of Biological Chemistry, 1957
- THE SEPARATION OF SPHINGOLIPIDES BY ADSORPTION CHROMATOGRAPHYJournal of Biological Chemistry, 1956
- DETERMINATION OF CEREBROSIDESJournal of Biological Chemistry, 1955
- PHOTOMETRIC DETERMINATION OF FATTY ACID ESTER GROUPS IN PHOSPHOLIPIDESJournal of Biological Chemistry, 1955
- A MICROMETHOD FOR THE QUANTITATIVE PARTITION OF PHOSPHOLIPIDE MIXTURES INTO MONOAMINOPHOSPHATIDES AND SPHINGOMYELINJournal of Biological Chemistry, 1946
- Über die Reindarstelluug von Sphingomyelin aus Gehirn. [14. Mitteilung über Phosphatide]Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1940