T-lymphocyte response to H-2 mutants. I. Proliferation is dependent on Ly 1+2+ cells.

Abstract

The Ly phenotype of the T [thymus-derived] lymphocytes which proliferate in response to mutant H-2K and H-2D alloantigens in primary mixed lymphocyte culture was determined. Responder T cells proliferating in reciprocal cultures of H-2d(KdDd) and H-2da(KdDda) lymphocytes were typed Ly 2+ through selective depletion with specific alloantiserum plus complement. B6-Ly 1a lymphocytes proliferating in response to B6-H-2ba and B6-H-2bf stimulators were typed as Ly 1+2+ through similar analysis. These results were discussed with regard to their impact on views of lymphocyte differentiation and factors determining the identity of alloreactive lymphocytes.