Improvement of chromosomal DNA extraction from different yeast species by analysis of single preparation steps
- 15 September 1995
- Vol. 11 (11), 1027-1029
- https://doi.org/10.1002/yea.320111104
Abstract
A modified procedure is proposed for chromosomal DNA extraction based on a cell‐wall lytic enzyme never applied before in pulsed field gel electrophoresis. Protoplasting efficiency is retained under very challenging conditions for enzyme activity, such as those required for non‐Saccharomyces yeasts often characterized by cell walls highly resistant to lysis.Keywords
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