Lysosomal and Non-Lysosomal Pathways of Intracellular Insulin Degradation in Isolated Rat Hepatocytes
- 1 January 1981
- journal article
- research article
- Published by S. Karger AG in Hormone Research
- Vol. 15 (4), 252-262
- https://doi.org/10.1159/000179464
Abstract
The amount of 125I-insulin associated with freshly isolated hepatocytes was increased 50% in the presence of 0.2 mM chloroquine (CQ) after 2 h of incubation. The degradation of insulin by the hepatocytes incubated with CQ was significantly diminished as compared with control cells. Hepatocytes incubated with 125I-insulin in the presence of CQ showed a slower rate of ligand dissociation than control cells. More TCA-precipitable and less TCA-soluble material appeared in the dissociation buffer of CQ-treated cells. However, CQ inhibited only 25-35% of intracellular insulin degradation. Non-lysosomal intracellular insulin degradation appears to be responsible for the remaining portion of the ligand degradation by isolated hepatocytes.Keywords
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