The Distribution of Lipids in the Bovine Lens and Cornea

Abstract

As a result of biochemical analyses of lipids in the bovine lens and cornea, Krause¹and, more recently, D'Asaro et al.²have been able to show that phospholipids form a major constituent of the lipid fractions from the bovine lens and cornea. In the light of such biochemical information it is naturally of interest to determine the histochemical picture of lipid and phospholipid distribution in these two avascular structures. Apart from Wislocki's histochemical study on the anterior segment of the eye of the rhesus monkey,³in which reference is made to the distribution of Sudan blackpositive material in the cornea and lens, no other attempt to localize lipid in these tissues on a cytological level seems to have been made. Furthermore, it has been recently established that an affinity for Sudan black may, on occasion, also characterize certain nonlipid substances⁴; additional tests are therefore required before