Uptake of Isolated Chloroplasts by Mammalian Cells
- 12 September 1969
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 165 (3898), 1128-1131
- https://doi.org/10.1126/science.165.3898.1128
Abstract
Mouse fibroblasts (L cells) in suspension culture incorporated isolated chloroplasts of spinach and African violets and isolated mitochondria of chicken liver. The organelles resided in the cell cytoplasm and were not contained in vacuoles or digestion vesicles. Green cells divided like normal cells. Green chloroplasts were followed for five cell generations or 5 days, at which time hybrid cells were greatly outnumbered by nongreen progeny cells. The ingested chloroplasts retained their structural integrity as determined by electron microscopy of organelles and hybrid cells and by analysis of photochemical activity and DNA in chloroplasts reisolated from cells after 1 or 2 days in culture.Keywords
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