Combined Liquid Chromatography-Mas Spectrometry for Microscale Structural Studies of Carbohydrates

Abstract

Combined HPLC-thermospray was spectrometry of mono-and disaccharides, 1-0-methylglycosides and 0-permethy mono - through tetrasaccharicdes has been studied to asess the potential role of thermospray ionization for microscale structural studies of saccharides and glycoconjugates, and for high sensitivity detection of liquid chromatographic effluents. Using NH₄CO₂H as evaluant for reversed-phase HPLC, abundant MNH₄ ⁴ ions are formed form monosaccharides and mono-and per-methylated saccharides, and are suitable formonitoring sub-nanogram constituents in HPLC effluents. Detection of 100 pg (0.5pmole) of 1-0-methylhexopy-ranosides with signal/noise> 10 is demonstrated.