Dependence of melittin structure on its interaction with multivalent anions and with model membrane systems*

Abstract

The conformation and the aggregation state of melittin were investigated in aqueous solutions having different pH values and ionic composition and upon interaction with phospholipids. While circular dichroism could only show the existence, in aqueous solution, of a random-coil or of a right-handed helical conformation, high resolution 1H and 13C NMR spectra, together with the results of photon correlation spectroscopy, produced evidence in favor of many different well-defined structural states, depending on anion concentration and change and on pH. In pure water at neutral pH melittin appeared to exist as a flexible random-coil monomer; in dilute NaCl a monomeric form was found which was still essentially unordered but presented a pronounced rigidity of structure and could be approximated to a prolate ellipsoid. When divalent anions were present (or when high ionic strengths were reached even with monovalent anions) melittin molecules associated into a compact disc-like tetramer. 31P NMR established correlations between the binding of phosphate ions and the variations in the structure or in the aggregation state of the polypeptide chains. At alkaline pH a helical tetramer was also found, different from that formed in the presence of divalent anions at neutral pH. Upon binding to phospholipids, melittin molecules could be visualized, similarly to what happens in aqueous phosphate solutions, as consisting essentially of a bent right-handed helix, with a grouping of polar residues alone 1 face of the molecule. The Glu and Lys residues were strongly immobilized, while there was no NMR evidence for any self-aggregation of the peptide; the ability of melittin to induce dichromate efflux from phospholipid vesicles was higher when the peptide was in the monomeric state than in the tetrameric one.