Herbicide-tolerant tobacco mutants selected in situ and recovered via regeneration from cell culture

Abstract
SUMMARY: The herbicides Bentazone and Phenmedipharm kill the leaves of intact tobacco plants but do not affect callus cultures. Tolerant mutants were isolated by treating leaves of previously γ-irradiated haploid plants with herbicide then excising and culturing the green herbicide-resistant cell clones on the otherwise yellowed leaves. Among plants subsequently regenerated were a total of ten stable independently isolated mutants. Sexual crosses show these ten represent four Bentazone and two Phenmedipharm loci; all mutants were recessive to wild type.