Notizen: In vivo Metabolism of [4-13C]Phenacetin in an Isolated Perfused Rat Liver Measured by Continuous Flow 13C NMR Spectroscopy

Abstract

Metabolism, [4-¹³C]Phenacetin, Continuous Flow ¹³C NMR Continuous flow ¹³C NMR spectroscopy has been used for the first time to monitor the metabolism of a ¹³C labeled drug in an isolated liver. Continuous and almost immediate information on the metabolite formation could be obtained using ¹³C labeled phenacetin without alteration of the biological system. The data are consistent with those observed by conventional techniques (HPLC, aliquot ¹³C NMR measurements). From the biological point of view the sensitivity of continuous flow ¹³C NMR spectroscopy is still low (10^-3 ᴍ). The results presented demonstrate however that non-invasive and non-radio-active real time monitoring of drug metabolism in intact organs is possible.