The assay of uridine diphosphoglucose dehydrogenase activity: Discrimination from xanthine dehydrogenase activity
- 1 April 1991
- journal article
- research article
- Published by Wiley in Cell Biochemistry and Function
- Vol. 9 (2), 103-110
- https://doi.org/10.1002/cbf.290090207
Abstract
The biochemical and quantitative cytochemical assays of the activity of uridine diphosphoglucose dehydrogenase (UDPG-D) have produced perplexing results. It is now shown that the perplexity may be due to the possibility that the coenzyme (NAD) required for UDPG-D activity, may be acting as a substrate for a second dehydrogenase, namely xanthine dehydrogenase, which may utilize NAD as its substrate. The activity of UDPG-D can be distinguished selectively by the pH of its optimal activity and by decreasing the concentration of the coenzyme used in the assay.Keywords
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