CELL-DAMAGE RESULTING FROM THE LABELING OF RAT LYMPHOCYTES AND HELA S3 CELLS WITH IN-111 OXINE

Abstract

Rat thoracic-duct lymphocytes and HeLa S3 [human cervical carcinoma cell] cells were labeled in vitro with different amounts of In-111 oxine. The labeled rat lymphocytes were tested for their ability to recirculate normally in syngeneic rats; the labeled HeLa S3 cells for their ability to divide to form colonies in tissue culture. Both cell types behaved normally when labeled with small amounts of In-111 oxine but at higher doses were obviously damaged. The HeLa S3 cells provided evidence that this damage was primarily radiation-induced. Limitations may be necessary on the use of In=111 oxine as a cell label for clinical purposes.