Affinity Chromatography of Human Fibroblast Interferon (IFN-beta1) by Monoclonal Antibody Columns
- 1 April 1983
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 64 (4), 905-910
- https://doi.org/10.1099/0022-1317-64-4-905
Abstract
Summary Eight hybridomas secreting monoclonal antibodies specific to human fibroblast interferon (IFN-β1) were identified by a new screening procedure based on immune precipitation with a second antibody and recovery of interferon activity from the immune precipitate. Immunoadsorbents were prepared from these monoclonal antibodies and used for purification of IFN-β1. Electrophoretically pure IFN-β1, having a specific activity of 3 × 108 to 6 × 108 units/mg was obtained by a single passage of partially purified IFN-β1 on these immunoadsorbents.Keywords
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