FACTORS THAT INFLUENCE THE PASSAGE OF ASCORBIC ACID FROM SERUM TO CELLS IN HUMAN BLOOD

Abstract

After addition of ascorbic acid to defibrinated human blood, its concs. were followed in serum separated at once and in serum left in contact with cells. At 37[degree]C, ascorbic acid was found to enter the blood cells. This transfer is self-terminative and occurs under an atmosphere of air in which ascorbic acid is lost, as well as under an atmosphere of nitrogen, in which deterioration is prevented in serum and in whole blood. With serum conc. raised to 100-120 mg. per liter, the ratio [image] remained well above unity. The passage of ascorbic acid into the cells appears to be associated with some metabolic activity since it was never observed at lower temps. The rate of transfer of ascorbic acid is enhanced by avoiding blood sedimentation.