Cloning of the V‐ATPase subunit G in plant: functional expression and sub‐cellular localization1
- 23 October 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 437 (3), 287-292
- https://doi.org/10.1016/s0014-5793(98)01252-6
Abstract
A 13-kDa tobacco plasma membrane protein was isolated from two-dimensional electrophoresis gels. After microsequencing, RT-PCR techniques and cDNA library screening allowed for the cloning of two cDNAs. These cDNAs encoded for the subunit G of the vacuolar H+-ATPase, the first one identified in plants. Analysis of mRNA distribution showed a maximum level in the leaves and in the stem of the apical part of the tobacco plant. Heterologous functional complementation of the yeast mutant (Δvma10::URA3) was achieved with the two cDNAs. After fractionation of microsomal membranes on linear sucrose gradient, Western blots were performed using antibodies against recombinant protein and three peaks were identified: one which comigrated with the tonoplast marker and the others at slightly higher density corresponding to endoplasmic reticulum and to plasma membrane fractions.Keywords
This publication has 41 references indexed in Scilit:
- Construction of a directory of tobacco plasma membrane proteins by combined two‐dimensional gel electrophoresis and protein sequencingElectrophoresis, 1997
- Auxin-Induced Differential Sensitivity of the H+-ATPase in Plasma Membrane Subfractions from Tobacco CellsBiochemical and Biophysical Research Communications, 1996
- Purification, sequencing and functions of calreticulin from maizeJournal of Experimental Botany, 1995
- Expression of Two Related Vacuolar H+-ATPase 16-Kilodalton Proteolipid Genes Is Differentially Regulated in a Tissue-Specific MannerPlant Physiology, 1995
- Isolation of Multiple cDNAs Encoding the Vacuolar H+-ATPase Subunit B from Developing Cotton (Gossypium hirsutum L.) OvulesPlant Physiology, 1994
- Two cDNA Clones Encoding Isoforms of the B Subunit of the Vacuolar ATPase from Barley RootsPlant Physiology, 1994
- Vacuolar H+-ATPase 69-Kilodalton Catalytic Subunit cDNA from Developing Cotton (Gossypium hirsutum) OvulesPlant Physiology, 1993
- Dissociation and Reassembly of the Vacuolar H+-ATPase Complex from Oat RootsPlant Physiology, 1992
- Calcium Transport in Sealed Vesicles from Red Beet (Beta vulgaris L.) Storage TissuePlant Physiology, 1987