The inactivation of animal amylase by plant paralysers and the presence of inactivating substances in solutions of animal amylase

Abstract

Salivary and pancreatic amylase were studied. Plant sistoamylase (SA) inactivated not only plant but also animal amylase. Dried SA inactivated amylase more powerfully than undried. In the place of origin of amy-lases as well as in their solutions, natural paralyzers, the so-called animal SA, were found. The system SA-amylase was thus formed, in which the amylase was partly inactivated. Usually ca. 1/3 of the salivary and ca. 1/5 of the pancreatic amylase was not adsorbed. In the system plant or animal SA-animal amylase, in which the amylase was inactivated, the amylase could be reactivated, except for certain forms of dried SA by the aid of eluting substances of which the best known was peptone. The increase of amylolytic action of amylase on the addition of certain substances was explained as an elution of amylase.