Integration, transcription, and control of a Drosophila heat shock gene in mouse cells.
- 1 November 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (11), 7038-7042
- https://doi.org/10.1073/pnas.78.11.7038
Abstract
Mouse L cells [neoplastic fibroblast] were transformed with a cloned 3.6-kilobase (kb) segment of D. melanogaster DNA carrying the 2.25-kb transcribed sequence for the Drosophila 70,000-dalton heat shock protein (hsp70) and 1.1 kb and 0.2 kb for 5'' and 3'' flanking DNA, respectively. Heat shock of 1 of 3 such transformed cell lines containing multiple copies of the intact Drosophila segment induced the abundant accumulation of transcripts of the Drosophila gene, with correct or nearly correct 5'' and 3'' termini. Diverse eukaryotes, including vertebrates, have heat shock systems similar to that studied extensively in Drosophila. The signals for heat shock transcription and the chromosomal sites with which they interact were highly conserved in evolution and that the regulatory sequences controlling transcription of the gene for hsp70 lie within the 3.6-kb Drosophila segment.Keywords
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